novel apexification method in a non-vital tooth with an open apex: a case report.

many materials have been introduced for apexification each having their own advantages and disadvantages. this case report aims to present a new method of apexification using a combination of deproteinized bovine bone mineral (dbbm) and enamel matrix derivative (emd). after irrigating the canal of the maxillary right canine with 2.5 % sodium hypochlorite, a mixture of bio-oss and emd was packed into the apical region for formation of an apical barrier and the canal was obturated by thermoplastic gutta percha technique with ah26 sealer; coronal seal was achieved by resin bonded composite. the size of the periapical lesion decreased significantly after 3, 6, 12 and 18-months. the patient had no radiographic signs or clinical symptoms at 24-month follow up and complete maturation of the apex and healing of the periapical bone were achieved.